Function:
The t-test tool is used to determine if two groups of data are significantly different from each other.The t-test is any statistical hypothesis test in which the test statistic follows a Student's t-distribution under the null hypothesis.
Input:
Attention:Input tab delimited text files with headers. If your data is not TAB delimited, you can use Excel to convert it.
(1) Different analysis table file:Input a tab delimited text file with headers. The first row is sample names and the first column is variable name. Values in the table are variable value.Each group should have 3 samples at least, and multiple groups of samples should be compared in pairs
(2) Comparison file:Input a tab delimited text file where a row represents a comparison with the second column versus the first column.
(3) Grouping file:Input a tab delimited text file where sample names in the first column and group names in the second column
Parameters:
(1) t-test type: Unpaired t-test or paired t-test. Paired samples t-tests typically consist of a sample of matched pairs of similar units, or one group of units that has been tested twice.。
(2) Test value: you can choose P value or Q value for test value. Q value (false discovery rate, FDR) is calculated by FDR correction of P value.
(3) Threshold for P value or Q value: Normally, the threshold for differential expression analysis is 0.05. You can also set the threshold according to your experiment, like 0.01 or 0.001.
(4) Threshold of fold change: The default of is 1, which means you don't think about fold change.
Graph:The default is histogram, the optional graphs are volcano plot and heat map.
diff_stat.xls: statistical table of differential variable numbers
diff_stat.pdf/png: differential variable histogram between comparison groups (vector/scalar figure)
A-vs-B.Ttest.all. xls: Differentially variable values table
A-vs-B. Ttest.filter.xls: Significantly differentially variable values table
A-vs-B.volcano.pdf/png: Differentially variable values volcano plot (vector/scalar figure)
A-vs-B.heatmap.pdf/png: Differentially variable values volcano plot (vector/scalar figure)
Example: Different analysis table file Comparison file Grouping file
Result graph:(The default is histogram, the optional graphs are volcano plot and heat map)
some result graphs: