Function：
The t-test tool is used to determine if two groups of data are significantly different from each other.The t-test is any statistical hypothesis test in which the test statistic follows a Student's t-distribution under the null hypothesis.
Input：
Attention：Input tab delimited text files with headers. If your data is not TAB delimited, you can use Excel to convert it.
（1） Different analysis table file：Input a tab delimited text file with headers. The first row is sample names and the first column is variable name. Values in the table are variable value.Each group should have 3 samples at least, and multiple groups of samples should be compared in pairs
（2） Comparison file：Input a tab delimited text file where a row represents a comparison with the second column versus the first column.
（3） Grouping file：Input a tab delimited text file where sample names in the first column and group names in the second column
Parameters：
（1） t-test type: Unpaired t-test or paired t-test. Paired samples t-tests typically consist of a sample of matched pairs of similar units, or one group of units that has been tested twice.。
（2） Test value: you can choose P value or Q value for test value. Q value (false discovery rate, FDR) is calculated by FDR correction of P value.
（3） Threshold for P value or Q value: Normally, the threshold for differential expression analysis is 0.05. You can also set the threshold according to your experiment, like 0.01 or 0.001.
（4） Threshold of fold change: The default of is 1, which means you don't think about fold change.
Graph：The default is histogram, the optional graphs are volcano plot and heat map.
diff_stat.xls： statistical table of differential variable numbers
diff_stat.pdf/png： differential variable histogram between comparison groups (vector/scalar figure)
A-vs-B.Ttest.all. xls： Differentially variable values table
A-vs-B. Ttest.filter.xls： Significantly differentially variable values table
A-vs-B.volcano.pdf/png： Differentially variable values volcano plot (vector/scalar figure)
A-vs-B.heatmap.pdf/png： Differentially variable values volcano plot (vector/scalar figure)
Example： Different analysis table file Comparison file Grouping file
Result graph：（The default is histogram, the optional graphs are volcano plot and heat map）
some result graphs：
