Function:
UniProtKB/Swiss-Prot is the manually annotated and reviewed section of the UniProt Knowledgebase (UniProtKB). It is a high quality annotated and non-redundant protein sequence database, which brings together experimental results, computed features and scientific conclusions.
We use DIAMOND software to align the query sequences to the Swiss-Prot protein database to find the proteins with highest sequence similarity.
Input：
paste or upload a nucleic or protein sequence in FASTA format.
Output:
① alignment result table
② alignment statistic pie chart ( alignment number pie chart and E-value distribution pie chart)
Output:
① alignment result table
Headers:
Query_id the ID of the query sequence
Subject_id the ID of the subject sequence
Identity(%) the alignment similarity (percentage)
Align_length alignment length
Mis_match number of mismatch
Gap number of Gap
Query_start the start site of the aligned query sequence
Query_end the end site of the aligned query sequence
Subject_start the start site of the aligned subject sequence
Subject_end the end site of the aligned subject sequence
E_value E value of alignment (the smaller the E value, the more the reliability)
Score the score of alignment (the higher the score, the more the reliability)
Subject_annotation description of Swiss-Prot subject sequence
② alignment number pie chart
We count up the aligned sequences number and show the result with a pie chart.
③ E-value distribution pie chart
E value is the expected value of the alignment. The smaller the E value, the more the reliability. We divided the E value into five ranges and show the number with a pie chart.
