NR Annotion





choosefile  example








The NR nucleotide database maintained by NCBI as a target for their BLAST search services is a composite of GenBank, GenBank updates, and EMBL updates.Non-redundant: Entries with absolutely identical sequences have been merged.

Input:

paste or upload a nucleic or protein sequence in FASTA format.
Output:
1. alignment result table
2. alignment statistic pie chart ( alignment number pie chart and E-value distribution pie chart)

example

Output:
1. alignment result table
Headers:
Query_id                               the ID of the query sequence
Subject_id                   the ID of the subject sequence
Organism Species(OS)        the species name of the subject sequence
Gene Name(GN)             the gene name of the subject sequence
Identity(%)                 the alignment similarity (percentage)
Align_length                alignment length
Mis_match                 number of mismatch
Gap                            number of Gap
Query_start               the start site of the aligned query sequence
Query_end                the end site of the aligned query sequence
Subject_start              the start site of the aligned subject sequence
Subject_end               the end site of the aligned subject sequence
E_value                   E value of alignment (the smaller the E value, the more the reliability)
Score                     the score of alignment (the higher the score, the more the reliability)
Subject_annotation          description of nr subject sequence

2. alignment number pie chart
We count up the aligned sequences number and show the result with a pie chart.
E-value distribution pie chart
E value is the expected value of the alignment. The smaller the E value, the more the reliability. We divided the E value into five ranges and show the number with a pie chart.