CAZy annotation





choosefile  example






CAZy database introduction

CAZy, Carbohydrate-Active enZYmes Database, a specialist database dedicated to the display and analysis of genomic, structural and biochemical information on carbohydrate-active enzymes, related to the breakdown, biosynthesis and/or modification of glycoconjugates, oligo- and polysaccharides. The enzymes are divided into 5 classes and 1 associated module according to the protein domain structure and amino acid sequences, including Glycoside Hydrolases (GHs), Glycosyl Transferases (GTs), Polysaccharide Lyases (PLs), Carbohydrate Esterases (Ces), Auxiliary Activities (Aas)and Carbohydrate-Binding Modules (CBMs).

Software DIAMOND  was used for sequences mapping and annotation based on database, with default parameters.

Input files

fasta file of nucleic acid or amino acid query sequences.

Results

1. Mapping and annotation results

1)Annotation results

2)Statics of A level (counts of 5 classes and 1 model)

3)Statics of B level (gene list of subclasses)

2. Statistics about 5 classes and 1 module.

3. Statistics of alignment

(1) showing the distribution of mapped and unmapped results.

(2) showing the distribution of E-values.



Example file

Results

1. Mapping and annotation results

1)Annotation results

Query_id :ID of query sequences

Query_length:the length of input sequences

Query_start :the start position of query sequences covered by alignment

Query _end :the end position of query sequences covered by alignment

Subject_id :ID of mapped sequences in database

Subject_start:the start position of subject sequences covered by alignment

Subject_end:the end position of subject sequences covered by alignment

Identity(%) :identity of alignment (percentity)

Positive:counts of  positive-scoring matches(Base or amino acid

Gap:number of gaps

Align_length:the length of sequences covered by alignment

Score:Score of alignment, the higher the better

E_value :Expcet values of alignment, the lower the better

Subject_annotation: CAZy classification of subject


2)Statics of A level (counts of 5 classes and 1 model)



class:abbreviation of classes and modules.
class name:name of classes and modules.
count :number of queries in each class and module.

3)Statics of B level (gene list of subclasses)



2. Statistics of CAZy levels

Statistics about 5 classes and 1 module.

3. Statistics of alignment

(1)showing the distribution of mapping and unmapping results.

(2)showing the distribution of E-values.